There are many uses of rDNA technology in medicine, industry, agriculture, food, baking, etc. Five applications of rDNA technology in medicine. This chapter looks at some of the tools scientists use in carrying out research of recombinant DNA. Cloning is undertaken in order to obtain the clone of one particular gene or DNA sequence of interest. Recombinant DNA (rDNA) technology refers to the process of joining DNA molecules from two different sources and inserting them into a host organism, to generate products for human use. Production of human growth hormone (hGH) by recombinant DNA technology. MAIN STEPS INVOLVED IN MOLECULAR CLONING USING RECOMBINANT TECHNOLOGY. Recombinant DNA technology combines DNA from different sources to create a different sequence of DNA. The next step after cloning is to find and isolate that clone among other members of the library (a large collection of clones). This "sticky ends" from two different DNA molecules can hybridize together; then the nicks are sealed using ligase. When this recombinant vector is inserted into E. coli, the cell … Recombinant DNA technology development and applications B. Recombinant DNA refers to the creation of new combinations of DNA segments that are not found together in nature. Let us see them in separate parts below. Genetic Engineering and Recombinant DNA Technology Diversity of life is based on the differences in genes (DNA) 1 gene = 1 protein = 1 function? It looks at some of the enzymes and other fundamental tools for working with DNA and cells that are used to manipulate and analyze DNA, including determining its sequence; to clone DNA; and to analyze proteins. The gene for human growth hormone (hGH) is isolated from human pituitary gland. Vitamins, antibiotics, enzymes, anticancer drugs, interferons, etc. are produced using this technology. The isolation and manipulation of genes allows for more precise genetic analysis as well as practical applications in medicine, agriculture, and industry. This process involves multiple steps that have to proceed in a specific sequence to generate the desired product. Recombinant DNA Technology- Steps, Applications and Limitations. As recombinant DNA technology advances, technique precision must be balanced by ethical concerns. Applications of Recombinant DNA technology. – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 45c32e-NmI2Y The result is recombinant DNA. Recombinant DNA technology refers to the joining together of DNA molecules from two different species that are inserted into a host organism to produce new genetic combinations that are of value to science, medicine, agriculture, and industry. Recombinant DNA technology is based primarily on two other technologies, cloning and DNA sequencing. CUTTING AND LIGATION OF FRAGMENTED DNA FROM DONOR AND FROM THE PLASMID….. STEPS IN DETAIL . Recombinant DNA technology is used in a wide range of applications from vaccine production to the production of genetically engineered crops.