In order to determine the accuracy and simplicity of the new test, it was compared with the two more standard … • Acetoacetic acid (present in urine) and sodium nitroprusside (in reagent strip) in alkaline medium gives purple color. Acetoacetic acid reacts with sodium nitroferricyanide and glycine in an alkaline medium to produce a violet-to-purple colored complex. Ketones are first deprotonated with alkali or glacial acetic acid to form carbanion species. This test … This simplified nitroprusside test has a reading time of only 15 sec. Testing for ketone bodies is based on a nitroprusside reaction. Nitroprusside test is commonly used to detect the ketones in the urine. Principle: This test is specific for Cys which has a free sulfhydryl group (-SH) that is able to react with the nitroprusside in the presence of excess ammonia (NH4OH). Principle: sodium nitroprusside reaction (nitroferricyanide) is used to detect ketones in urine. A standardized dip-and-read test for the detection of ketone bodies is described. This test, like the other nitroprusside tests, depends on the reaction between acetoacetic acid and nitroprusside to produce a colored complex. The colors range from beige or buff-pink color for a … This then reacts with the sodium nitroprusside reagent to form a deep red color complex which … The test is of value in assessing the severity of human ketosis since it can be used to determine the level of ketone bodies in both urine and serum. Ketones can be detected in serum and urine using the nitroprusside reaction on diagnostic strips for use at the patient's bedside or in the clinical laboratory. Principally, the free thiol group of Cys gives a red color with sodium nitroprusside in the presence of NH 4 OH. • The test … TEST PRINCIPLE Ketone: This test is based on the reaction of acetoacetic acid with sodium nitroprusside in a strongly basic medium. (g) Sodium Nitroprusside Test: Dissolve sodium nitroprusside in distilled water in a clean test tube. Add 1ml of the given organic compound to be tested. Three ketones are produced in DKA: two ketoacids (β-hydroxybutyrate and acetoacetate) and the neutral ketone, acetone. Shake well and add sodium hydroxide solution dropwise. The … If there is the appearance of red colour then the presence of ketone … Recently the nitroprusside test for urine ketones has been made available as a stick or dip test with a preparation called Ketostix.